Description
Description: The cell proliferation assay reagent alamarBlue is designed to provide a rapid and sensitive measure of cell proliferation and cytotoxicity in various human and animal cell lines, bacteria and fungi. alamarBlue is an indicator dye, that incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and changes colour in response to the chemical reduction of growth medium, resulting from cell growth. The alamarBlue cell proliferation assay reagent is designed to quantitatively measure the proliferation of various human and animal cell lines, bacteria and fungi. Full cell proliferation assay instructions can be found atwww.abdMyBioSource.com/uploads/IFU/MBS238967.pdf For further information and Technical help about alamarBlue, the cell proliferation assay reagent, please visit www.abdMyBioSource.com/alamarBlueThis site includes: Frequently Asked QuestionsExample calculationsProduct-related references
Test Principle: Cell proliferation assay. Growing cells cause a chemical reduction of alamarBlue.
Continued growth maintains a reduced environment. (fluorescent, red).
Inhibition of growth maintains an oxidized environment. (non-fluorescent, blue).
Data may be collected using either fluorescence-based or absorbance-based instrumentation.
Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength.
Absorbance is monitored at 570nm and 600nm